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==Mike Kelly talk=====Introduction===
* Part of the standard repertoire of biological research techniques
* Look at heterogeneity
* interrogate potential mechanisms at cellular resolution
===Sample Prep===
# Solid tissue
# dissociation
* droplet-based gel cell barcode
* plate-based sc-Seq using flow cytometry - allows gating to target cells of interest
* What is the RNA quality? We don't know. Fixed cells? time since extraction can have a big affect on data quality
** full length transcript allows get isoform analysis
* 10xgenomics prep guidelines
** run all the samples together in the sequencing run, reduces technical variation for biological replicates, more statistical power
===Analysis Toolkits===
* Loupe Cell Browser (free from 10x genomics)
* Partek flow (14-day free trial)
* [https://nih-irp-singlecell.github.io NIH Single Cell Community]
===Public datasets===
* [https://www.10xgenomics.com/resources/datasets/ 10x genomics datasets]
==Nan Ping talk==
* innate immunity - non specific
* adaptive immunity - b cells antibody, CD4 hemper cells and CD8 killer cells
* functional decline in age
* naive t cells - then expansion and proliferation into effector cells, becomes memory cells. 7 different types of t-cells known
* What type of lymphocyes are reduced by age - naive
* how many CD8 sub populations are there?
* Age increase in number of cells, or expression level within a cell, or both?
